An Amperometric Biosensor for the Determination of Organophosphorus Pesticides

Authors: Azmi TELEFONCU

Abstract: Organophosphorus pesticides are inhibitors of cholinesterase and this enzyme is used as a monitoring system for pesticides containing organophosphorus compounds. The decrease in activity of cholinesterase is monitored by the choline sensor and is correlated to the concentration of pesticide present in solution. According to this observation a biosensor for pesticides was determined. This biosensor consists of a Clark oxygen electrode, modified with cellulose acetate and dialysis membranes. Between the two membranes there are two immobilized enzymes: acetyl-cholinesterase and choline oxidase.


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