Authors: GÜLAÇTI TOPÇU, MEHMET ÖZTÜRK, TUBA KUŞMAN, AYŞE ASLI BARLA DEMİRKOZ, UFUK KOLAK, AYHAN ULUBELEN
Abstract: The hexane and dichloromethane extracts, obtained by re-extraction of the methanol extract of Salvia fruticosa Mill., afforded 7 diterpenoids (carnosol, carnosic acid, carnosic acid 12-methyl ether, rosmadial, isorosmanol, ferruginol, and manool), 4 triterpenoids (\alpha -amyryltetracosanoate, oleanolic acid, ursolic acid, and erythrodiol), a steroid (3-acetylsitosterol), and a flavone (salvigenin). The galls (apples) of the collected plant were separately extracted with hexane to afford fatty acids composed mainly of oleic acid (29\%), palmitoleic acid (29\%), and stearic acid (23.20\%), which exhibited high anticholinesterase activity, particularly against BChE. The essential oil, obtained from the aerial parts of the plant, exhibited high AChE inhibitory activity, consisting of mainly 1,8-cineol (58.89\%). The antioxidant activity according to 6 complementary tests and anticholinesterase potential of the methanol extracts and triterpenoids \alpha-amyryltetracosanoate, oleanolic acid, ursolic acid, and sitosterol acetate were also investigated, and methanol extract exhibited the highest antioxidant and anticholinesterase activity, surpassing the other tested extracts and pure compounds.
Keywords: Lamiaceae, Salvia fruticosa, secondary metabolites, abietane diterpenoids, triterpenoids, essential oil, fatty oil, antioxidant, anticholinesterase
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