Authors: CHERUTH ABDUL JALEEL, RAGUPATHI GOPI, PARAMASIVAM MANIVANNAN, RAJARAM PANNEERSELVAM
Abstract: Antioxidant responses were analysed in Catharanthus roseus (L.) G.Don. under 0, 50 and 100 mM NaCl in order to investigate the plant´s protective mechanisms against long-term salt-induced oxidative stress. The NaCl treatments were repeated in 4 different stages of growth, i.e. 30, 45, 60 and 75 days after sowing (DAS). The plants were uprooted randomly 90 DAS and the non-enzymatic and enzymatic antioxidant potentials were analysed. High salinity caused a decrease in reduced glutathione (GSH) and an enhancement in total ascorbate (AA) content and the antioxidant enzyme and ascorbate peroxidase (APX, EC 18.104.22.168) activities. Moreover, salinity induced a significant decline in superoxide dismutase (SOD, EC 22.214.171.124) and peroxidase (POX, EC 126.96.36.199) activities. The changes found in catalase (CAT, EC 188.8.131.52) activities may be of great importance in the H_2O_2 detoxification mechanism under oxidative stress.
Keywords: Antioxidants, antioxidant enzyme, salinity, Catharanthus roseus
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