Authors: SATI UZUN, İSKENDER PARMAKSIZ, SERKAN URANBEY, SEMRA MİRİCİ, ERCÜMENT OSMAN SARIHAN, ARİF İPEK, MEHMET DEMİR KAYA, BİLAL GÜRBÜZ, NEŞET ARSLAN, CENGİZ SANCAK, KHALID MAHMOOD kHAWAR, SEBAHATTİN ÖZCAN
Abstract: An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of \alpha-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.
Keywords: Muscari muscarimi Medik., immature embryo, tissue culture
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