Plant Regeneration from Callus Culture of Clematis gouriana Roxb. – A Rare Medicinal Plant


Abstract: An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l^{-1} BAP and 0.1 to 0.5 mg l^{-1} NAA. The optimized callus induction occurred at the concentration of 1.0 mg l^{-1} BAP and 0.3 mg l^{-1} NAA. After initiation of callus, it was immediately transferred to MS medium containing 4.0 mg l^{-1} FAP and 0.5 mg l^{-1} indole-3-butyric acid (IBA). Upon longer incubation for about 2-6 weeks on the same culture medium initiation of shoot buds from the callus mass was observed and regeneration of plantlets with higher frequency (mean of 11.1 ± 0.23 shoots per explants) was noted. The microshoots rooted well on MS basal medium without growth regulators, as well as on medium supplemented with 0.5 mg l^{-1} IBA. Regenerated shoots formed complete plantlets on medium containing 0.5 mg l^{-1} IBA, and mature plants were established, acclimatized, and thrived in greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant.

Keywords: Clematis gouriana, medicinal climber, stem explant, plant regeneration

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